Post-translational protein modifications by members of the ubiquitin family are widely recognized as important regulatory control systems for a variety of biological pathways. Their influence on eukaryotic cellular metabolism is comparable to that of other modifi- tions such as phosphorylation, acetylation and methylation. The small ubiquitin-related modifier SUMO uses a conjugation and de-conjugation system closely related to that of ubiquitin itself; yet, the functions of the SUMO system are highly diverse and largely independent of the ubiquitin system. SUMO modification controls the activity of tr- scription factors and can influence protein stability, but it also contributes to nucleo-cy- plasmic transport, chromosome segregation and DNA damage repair. As a consequence, the SUMO system pervades virtually all areas of basic molecular and cell biology, and scientists from different backgrounds, including medical researchers, are likely to enco- ter SUMO in the course of their studies. This volume, SUMO Protocols, therefore aims at presenting a collection of methods relevant to SUMO research in order to make these tools available to biochemists, molecular and cell biologists as well as research-oriented clinicians not yet familiar with the system. In contrast to the ubiquitin system, which has been the subject of several reviews and methods collections, no practical compendium entirely devoted to SUMO has been p- lished.
Publisher: Humana Press Inc.
Number of pages: 324
Weight: 829 g
Dimensions: 260 x 193 x 23 mm
Edition: Softcover reprint of hardcover 1st ed. 2009
From the reviews:
"The modification of proteins by small ubiquitin-like modification (SUMO) is relevant to virtually all areas of molecular and cell biology. SUMO Protocols is a quite comprehensive volume describing various in vivo and in vitro techniques ... . The target audience is likely to be postdoctoral fellows and PhD students in laboratories not only dedicated to SUMO pathways but those beginning their initial forays in the field ... and regulated by SUMO. ... for those pursuing SUMO-specific modification this is a worthwhile addition to their bookshelves." (Peter O'Hare, Microbiology Today, July, 2009)