This book collects the most effective and cutting-edge methods and protocols for deriving and culturing human embryonic and adult stem cells-in one handy resource. This groundbreaking book follows the tradition of previous books in the Culture of Specialized Cells Series-each methods and protocols chapter is laid out exactly like the next, with stepwise protocols, preceded by specific requirements for that protocol, and a concise discussion of methods illustrated by data. The editors describe a limited number of representative techniques across a wide spectrum of stem cells from embryonic, newborn, and adult tissue, yielding an all-encompassing and versatile guide to the field of stem cell biology and culture. The book includes a comprehensive list of suppliers for all equipment used in the protocols presented, with websites available in an appendix. Additionally, there is a chapter on quality control, and other chapters covering legal and ethical issues, cryopreservation, and feeder layer culture. This text is a one-stop resource for all researchers, clinical scientists, teachers, and students involved in this crucial area of study.
Publisher: John Wiley and Sons Ltd
Number of pages: 360
Weight: 806 g
Dimensions: 258 x 184 x 22 mm
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