• Sign In / Register
  • Help
  • Basket0
The books you love, the emails you want
Time is running out, opt in before 25 May or you'll stop hearing from us
Yes Please
siRNA Design: Methods and Protocols - Methods in Molecular Biology 942 (Paperback)
  • siRNA Design: Methods and Protocols - Methods in Molecular Biology 942 (Paperback)

siRNA Design: Methods and Protocols - Methods in Molecular Biology 942 (Paperback)

Paperback 392 Pages / Published: 23/08/2016
  • We can order this

Usually despatched within 3 weeks

  • This item has been added to your basket

Check Marketplace availability

The discovery of RNA interference (RNAi) as a methodology for gene silencing has revolutionized biological research, providing an invaluable avenue for therapeutics, and small interfering RNA (siRNA) is the most common strategy utilized for enacting RNAi. siRNA Design: Methods and Protocols offers expertly crafted guidelines and protocols for the selection of siRNA targeting sequences, for the strategic incorporation of chemical modifications, and for advantageous structural modifications to the classic siRNA design. Protocols are provided for using endogenous cellular machinery to produce siRNA from optimized precursor short hairpin RNA (shRNA) and artificial microRNA (amiRNA) molecules. Strategies are also described for specific applications such as immunostimulatory siRNA that may provide therapeutic benefit against viral infections in mammals, the simultaneous targeting of multiple siRNAs, and siRNA-mediated crop virus resistance. The design of RNAi for gene silencing in embryonic, invertebrate, and plant systems requires a variety of unique approaches, several of which are described towards the end of this volume. Written for the highly successful Methods in Molecular Biology™ series, this work contains the kind of detailed description and implementation advice that guarantees successful results.

Authoritative and easy to use, siRNA Design: Methods and Protocols will provide researchers, educators, clinicians, and biotech specialists with a broad understanding of the issues in RNAi and how they can be overcome strategically through design.

Publisher: Humana Press Inc.
ISBN: 9781493959785
Number of pages: 392
Weight: 773 g
Dimensions: 254 x 178 x 21 mm
Edition: Softcover reprint of the original 1st ed. 201


From the reviews:

"This book edited by D.J. Taxman is a valuable collection of 20 chapters covering aspects from values for parameters used in tools for designing siRNAs to screening methods for evaluating the efficiency of the designed siRNA. ... Through style and scientific information the book is aimed at researchers in the RNAi field, but its accessible delivery of contents makes it a good starting point for younger researchers in this topic." (Irina Ioana Mohorianu, zbMATH, Vol. 1276, 2014)

You may also be interested in...

Biological Sequence Analysis
Added to basket
The Case Against Perfection
Added to basket
Genes, Cells and Brains
Added to basket
Starved for Science
Added to basket
Natural Products Isolation
Added to basket
Gene Cloning
Added to basket
The March of Unreason
Added to basket
Modern Dictionary of Biotechnology
Added to basket
Added to basket


Please sign in to write a review

Your review has been submitted successfully.