This text explores the fundamentals and the scope of protein engineering. It explains the design of novel proteins and the principles of protein structure, demonstrating how protein engineering experiments can be planned. It describes the techniques used in recombinant DNA and in vitro mutagenesis, including the principles involved in the manipulation of DNA and the use of expression vectors to produce engineered proteins. Finally, the applications of protein engineering are included, with particular reference to structure-function experiments and future trends.
Publisher: John Wiley and Sons Ltd
Number of pages: 130
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