Modification of target protein properties by reversible phosphorylation events has been found to be one of the most prominent cellular control processes in all organisms. Recent advances in the areas of molecular biology and biochemistry are presenting new possibilities for reaching an unprecedented depth and a proteome-wide understanding of phosphorylation processes in plants as well as in other species. The major goal of Plant Kinases: Methods and Protocols is to provide the experimentalist with a detailed account of the practical steps necessary for successfully carrying out each protocol in his or her own laboratory. Plant protein kinases specifically addressed in this volume are members of the plant MAP kinase cascade, cyclin- and Calcium-dependent protein kinases, and plant sensor and receptor kinases. Written in the highly successful Methods in Molecular Biology ™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Plant Kinases: Methods and Protocols will prove a useful laboratory companion to both novice and seasoned researchers by facilitating the practical work that will lead them to new and exciting insights in this dynamic field.
Publisher: Humana Press Inc.
Number of pages: 312
Weight: 836 g
Dimensions: 254 x 178 x 19 mm
Edition: 2011 ed.
From the reviews:"This book represents a compendium of practical protocols to experimentally address different aspects of plant kinase function and regulation. It is intended to provide a benchtop manual for newcomers to the field but also to present experimental alternatives for the more specialized and advanced user. ... this book compiles a wealth of information on how to study plant kinases and provides a useful set of instructions for a wide range of complementary techniques in this field." (Joerg Kudla, Journal of Plant Physiology, Vol. 169 (15), 2012)