It has been more than five years since the appearance of the first edition of Nitric Oxide Protocols. According to Medline, more than 32,500 publications dealing with nitric oxide have appeared in the primary literature in the int- vening time from 1999 to April 2004. These numbers attest to the continuing vitality of a field dealing with a deceptively simple, although extremely int- esting and promiscuous molecule, nitric oxide. Despite its apparent chemical simplicity, nitric oxide continues to amaze, with an extremely wide spectrum of functions and effects. In particular, the last few years have witnessed an explosion of nonclassical effects, involving the direct interaction of nitric - ide and related oxidized products with proteins to yield such novel combi- tions as nitrosylated and nitrated proteins. Another emergent area has been that of NO's clinical applications, including interesting developments related to the virus-mediated expression of nitric oxide synthase and the use of nitric oxide in gene therapy. In Nitric Oxide Protocols, I have gathered chapters dealing with the some of the latest methodologies for the measurement of nitric oxide.
Many of these deal with quantitation in biological fluids or tissues, and they cover a wide spectrum of technologies, including capillary electrophoresis, microcoaxial electrodes, the in vivo measurement of nitric oxide in exhaled air, confocal microscopy, gas chromatography-mass spectroscopy, in situ hybridization, and real-time polymerase chain reaction.
Publisher: Humana Press Inc.
Number of pages: 248
Weight: 1200 g
Dimensions: 235 x 155 x 15 mm
Edition: 2nd ed. 2004
From reviews of the first edition...
"...an excellent book...offers newcomers and experienced scientists a wealth of important practical information that will be of inestimable value both to newcomers and experienced research groups...an essential resource for every laboratory involved in NO-related work..." -Angewandte Chemie
"...a very useful handbook and definitely a must for researchers working at the bench in any field related one way or another to NO." -Psychoneuroendocrinology
"...This book is a must for laboratories with an interest in nitric oxide..." -British Journal of Anesthesia
"...will certainly find its way into many laboratories entering the exciting field of nitric oxide research. " -Quarterly Review of Biology