• Sign In
  • Help
  • My Basket0
Molecular Basis of Neurological Disorders and Their Treatment (Paperback)
  • Molecular Basis of Neurological Disorders and Their Treatment (Paperback)
zoom

Molecular Basis of Neurological Disorders and Their Treatment (Paperback)

(author), (author), (author), (author)
£197.00
Paperback 337 Pages / Published: 08/10/2012
  • We can order this

Usually despatched within 3 weeks

  • This item has been added to your basket

Check Marketplace availability

cytochemical techniques (ICC) which provide a useful adjunct to investigations by immunoblotting. A particular advantage of a cytochemical approach to the investiga- tion of mitochondrial disorders is that it allows the mosaic distribution of certain of these defects to be detected, whereas the tissue homogeniza- tion involved in conventional enzyme assays or immunoblotting precludes this. A further advantage of MEA or ICC is that only small amounts of tissue are needed, which is important since many of the affected patients are infants or small children. The main aim of this communica- tion is to outline ways in which these techniques can be used in the diagnosis and further investigation of mitochondrial disorders. Reference will be made not only to those situations in which MEA and ICC offer advantages over standard enzyme asays and immunoblotting but also to contexts in which the reverse applies. 4. 2 MATERIALS AND METHODS Muscle biopsies for cytochemical investigation were snap-frozen using isopentane cooled to - 150 DegreesC in liquid nitrogen. Samples were stored in heat-sealed polythene packets in the vapour phase of liquid nitrogen containers. 4. 2. 1 Microphotometric enzyme assays Frozen sections 8 Jlm thick were cut using a Reichert-J ung Frigocut cryostat microtome equipped with motor-driven cutting action to maintain maximal reproducibility of section thickness. Sections were picked up on microscope slides and air-dried for 15 min at room temperature.

Publisher: Springer
ISBN: 9789401053792
Number of pages: 337
Weight: 545 g
Dimensions: 235 x 155 x 18 mm
Edition: Softcover reprint of the original 1st ed. 199

You may also be interested in...

Headhunters
Added to basket
£11.99
Paperback
The Autistic Brain
Added to basket
Connectome
Added to basket
£9.99
Paperback
The Human Brain Coloring Book
Added to basket
The Domesticated Brain
Added to basket
Murderous Minds
Added to basket
£10.99
Paperback
The Origins of Virtue
Added to basket
Incognito
Added to basket
£9.99
Paperback
Mind Change
Added to basket
£9.99
Paperback
The Developing Mind, Second Edition
Added to basket
The Brain Book
Added to basket
£25.00
Hardback
The Private Life of the Brain
Added to basket
We Are Our Brains
Added to basket
Descartes' Error
Added to basket
£10.99
Paperback
The Conscious Mind
Added to basket
The Plastic Mind
Added to basket
£10.99
Paperback

Reviews

Please sign in to write a review

Your review has been submitted successfully.