When the last edition of this book was published in 2000, the field of proteomics was in its infancy. At that time, multidimensional liquid chromatographic separations were being introduced as an alternative to traditional gel-based techniques for separating complex protein and peptide mixtures prior to mass spectrometric detection. Today, this approach - referred to as shotgun proteomics - is considered routine for lar- scale global analyses of protein mixtures. Now in its adolescence, proteomics is fundamentally transforming biological and medical research. Much of this transformation can be attributed to technological advancements, particularly in mass spectrometry. Much wider accessibility of hi- resolution and mass measurement accuracy instrumentation in recent years has ini- ated a new revolution in the field by providing more reliable data and shifting the focus from cataloging proteins to precisely quantifying changes in protein abundance over time and in response to stimuli. Advanced mass spectrometers and novel ion d- sociation schemes such as electron transfer/capture dissociation make it possible to venture boldly into the maze of protein posttranslational modifications, which are an integral component of understanding functional proteomics in the spatial and t- poral domains. Another area that has benefited from these advancements is top-down proteomics, an emerging method essential for characterizing various protein variants that has potentially high impact in biomedical research.
Publisher: Humana Press Inc.
Number of pages: 470
Weight: 1029 g
Dimensions: 260 x 193 mm
Edition: 2nd ed. 2009
From the reviews of the second edition:
"This book ... covers a wide range of applications of modern mass spectrometry in peptide and protein analysis. ... Very useful is the presence of a final 'Notes' section within each chapter. These notes and laboratory tips help both beginners and experienced researchers to make their first steps into new fields ... . The well balanced mixture ... makes it a helpful resource for scientists working in analytical chemistry, biochemistry, biotechnology, and bioinformatics, and a valuable resource for everyone working in biologically oriented mass spectrometry." (Andreas Tholey, Analytical and Bioanalytical Chemistry, September, 2009)