Mammalian Cell Biotechnology: A Practical Approach - Practical Approach Series 76 (Paperback)M. Butler
Paperback Published: 25/07/1991
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The development of mammalian cell biotechnology has led to an extensive range of commercially-valuable prophylactic, diagnostic and therapeutic compounds which can now be produced routinely by cell culture. This book details the principles and practical techniques upon which this technological development is based. The ten chapters are designed to cover the basic principles and techniques which can be applied to maximize the productivity of cell cultures. Media optimisation and the use of serum-substitutes or growth factors are explained. Methods of genetic manipulation of cells are explored as well as the techniques for maximizing specific protein expression. Chapters are included to explain the design and control of animal cell fermentation processes and the means of extraction of desired products by downstream processing. Finally, a chapter is included to explain the strategies for the application of these techniques to the production of specific compounds which have commercial value. This book will be of value to both the research student of biotechnology in academic institutions and to the industrialist who needs to maximize productivity from mammalian cell processes. The detailed principles and techniques described in the book should advance the understanding and realization of high productivity from mammalian cell cultures.
Publisher: Oxford University Press
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