This book describes developments in the field of super-resolution fluorescence microscopy or nanoscopy. In 11 chapters, distinguished scientists and leaders in their respective fields describe different nanoscopy approaches, various labeling technologies, and concrete applications. The topics covered include the principles and applications of the most popular nanoscopy techniques STED and (f)PALM/STORM, along with advances brought about by fluorescent proteins and organic dyes optimized for fluorescence nanoscopy. Furthermore, the photophysics of fluorescent labels is addressed, specifically for improving their photoswitching capabilities. Important applications are also discussed, such as the tracking and counting of molecules to determine acting forces in cells, and quantitative cellular imaging, respectively, as well as the mapping of chemical reaction centers at the nano-scale.
The 2014 Chemistry Nobel Prize ® was awarded for the ground-breaking developments of super-resolved fluorescence microscopy. In this book, which was co-edited by one of the prize winners, readers will find the most recent developments in this field.
Publisher: Springer-Verlag Berlin and Heidelberg GmbH & Co. KG
Number of pages: 335
Weight: 718 g
Dimensions: 235 x 155 x 18 mm
Edition: Softcover reprint of the original 1st ed. 201
"This is the first comprehensive book that summarizes most of the superresolution imaging techniques, and as such it is an excellent resource for newcomers to the field. ... a unique and much needed resource that contains a detailed description of all the superresolution techniques and methods that led to the award of the Nobel Prize in Chemistry in 2014. It is a great first resource for anyone wanting to learn more about this rapidly evolving field of research." (Thomas Huser, Analytical and Bioanalytical Chemistry, Vol. 408, 2016)