Visit our Christmas Gift Finder
DNA Topoisomerase Protocols: Volume II: Enzymology and Drugs - Methods in Molecular Biology 95 (Hardback)
  • DNA Topoisomerase Protocols: Volume II: Enzymology and Drugs - Methods in Molecular Biology 95 (Hardback)
zoom

DNA Topoisomerase Protocols: Volume II: Enzymology and Drugs - Methods in Molecular Biology 95 (Hardback)

(editor), (editor)
£127.50
Hardback 334 Pages / Published: 20/10/2000
  • We can order this

Usually dispatched within 3 weeks

  • This item has been added to your basket
Beginning with the Escherichia coli ? protein, or bacterial DNA topoisomerase I, an ever-increasing number of enzymes have been identified that catalyze changes in the linkage of DNA strands. DNA topoisomerases are ubiquitous in nature and have been shown to play critical roles in most p- cesses involving DNA, including DNA replication, transcription, and rec- bination. These enzymes further constitute the cellular targets of a number of clinically important antibacterial and anticancer agents. Thus, further studies of DNA topology and DNA topoisomerases are critical to advance our und- standing of the basic biological processes required for cell cycle progression, cell division, genomic stability, and development. In addition, these studies will continue to provide critical insights into the cytotoxic action of drugs that target DNA topoisomerases. Such mechanistic studies have already played an important role in the development and clinical application of antimicrobial and chemotherapeutic agents. The two volumes of DNA Topoisomerase Protocols are designed to help new and established researchers investigate all aspects of DNA topology and the function of these enzymes. The chapters are written by prominent investigators in the field and provide detailed background information and st- by-step experimental protocols. The topics covered in Part I: DNA Topology and Enzymes, range from detailed methods to analyze various aspects of DNA structure, from linking number, knotting/unknotting, site-specific recombi- tion, and decatenation to the overexpression and purification of bacterial and eukaryotic DNA topoisomerases from a variety of cell systems and tissues.

Publisher: Humana Press Inc.
ISBN: 9780896035126
Number of pages: 334
Weight: 665 g
Dimensions: 235 x 155 x 20 mm
Edition: 2001 ed.


MEDIA REVIEWS
"This book completes a comprehensive collection of assays, including the weird and wonderful, that can be used to investigate the enzymology and drug inhibition of topoisomerases...I recommend any students about to undertake a PhD degree in this area to buy a copy. Even those who are well versed in the biology of topoisomerases should consider buying both volumes of this book, they will surely find some things they didn't know - I did!" - MicroBiology Today

"Described by expert experimentalists who have perfected the techniques, these reproducible methods include assays for enzyme-catalyzed DNA relaxation/supercoiling, DNA cleavage, DNA nicking, DNA decatentaion, and ATP hydrolysis." - Anticancer Research

"An unprecedented collection of cutting-edge experimental protocols for investigating the catalytic activities of DNA topoisomerases, as well as their specific interactions with topoisomerase-targeted antitumor and antibacterial drugs." -Tumori

You may also be interested in...

Endless Forms Most Beautiful
Added to basket
Mutants
Added to basket
£12.99
Paperback
River Out of Eden
Added to basket
The Cosmic Serpent
Added to basket
£8.99
Paperback
The Major Transitions in Evolution
Added to basket
BIOS Instant Notes in Genetics
Added to basket
Nature via Nurture
Added to basket
£10.99
Paperback
The Seven Daughters Of Eve
Added to basket
Genome
Added to basket
£10.99
Paperback
The Biology of Cancer
Added to basket
The Epigenetics Revolution
Added to basket
The Violinist's Thumb
Added to basket
£9.99
Paperback

Please sign in to write a review

Your review has been submitted successfully.