Calcium is arguably the most diverse and widespread of the intra-cellular second messengers, being involved in many mechanisms of cellular biochemistry, physiology and pathology, where it plays a key role in signal transduction. Hormones and other extra-cellular agents bring about many of their effects by causing changes in intra-cellular calcium concentration. This book provides a comprehensive description of current techniques for measuring intra-cellular calcium concentrations, including protocols for the use of fluorescent, bioluminescent and metallochromic indicators, and various other intra-cellular probes. Also outlined are methods for controlling and changing the calcium ion concentration in cells, as well as procedures used to study the important proteins involved in the membrane transport and intra-cellular binding of calcium.
Publisher: Oxford University Press
Number of pages: 448
Weight: 670 g
Dimensions: 235 x 156 x 25 mm
'This book is a collection of well-written chapters that focus on practical aspects of cellular Ca2+. The chapters on the methods for measuring [Ca2+]i are informative, and rich with experimental details that only experts working daily with this subject could provide. Lists of reagents required for each procedure are particularly helpful, and represent a direct way to focus the readers' attention on the most relevant issues.'
Trends in Cell Biology, Vol. 2, August 1992
'This is a book based on a great deal of practical experience which gives insights into associated problems and is thus extremely useful ... This book has to be strongly recommended for individual purchase due to the wealth of laboratory based protocols, which from the reviewer's brief experience have proved invaluable.'
F. McDonald, European Journal of Orthodontics, 15 (1993)
'this book will be useful for someone already working in this area who wishes to gain a working knowledge of the latest experimental procedures on cellular calcium'
H.E. Knaggs, Biochemical Education 21(4) 1993