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Capillary Electrophoresis of Carbohydrates - Methods in Molecular Biology 213 (Hardback)
  • Capillary Electrophoresis of Carbohydrates - Methods in Molecular Biology 213 (Hardback)

Capillary Electrophoresis of Carbohydrates - Methods in Molecular Biology 213 (Hardback)

(editor), (editor)
Hardback 318 Pages / Published: 14/01/2003
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Glycoconjugates such as glycoproteins and glycolipids play important roles in cell-cell interaction events, including development, differentiation, m- phogenesis, fertilization, inflammation, and metastasis. A number of reports have documented the association of unique oligosaccharide sequences to p- tein targeting and folding, and in mechanisms of infection, inflammation, and immunity. For glycoproteins, these glycan appendages are the result of extensive co- or post-translational modifications of the nascent proteins in the endoplasmic reticulum and in the Golgi apparatus. Although nucleic acids and proteins are copied from a template in a repeated series of identical steps using the same enzymes, complex carbohydrates are formed by the sequential actions of cellular glycosyltransferases that specifically recognize unique s- strates. The molecular biology of these transferases and other carbohydra- modifying enzymes is providing important insights on oligosaccharide recognition events. While it is acknowledged that the definition of the protein complement of cells and tissues (the so-called proteome) remains an enormous task in this postgenomic era, the characterization of all glycans produced by individual organisms (referred to as the glycome) presents an equally imp- tant challenge. This task is further complicated by the fact that oligosacc- rides cannot presently be cloned. These complex carbohydrates exist in a staggering diversity of structures, linkages, and branching, thus providing an exquisite molecular repertoire for cellular interactions.

Publisher: Humana Press Inc.
ISBN: 9780896038264
Number of pages: 318
Weight: 647 g
Dimensions: 235 x 155 x 19 mm
Edition: 2003 ed.

"...the book will be very valuable for researchers carrying out carbohydrate research, or planning to do so, and contemplating the best analytical approaches." -Pharmaceutical Research

"...this book provides a great deal of useful practical detail for CE analysis of carbohydrates for the experienced as well as the newcomer to the field." -Biomedical Chromatography

"...the book is a nice compilation of protocols for the analysis of carbohydrates by does contain all the essential procedures and methods needed to solve many problems." - Chromatographia

"...this book comprehensively summarizes knowledge on carbohydrate analysis by means of capillary electrophoresis." - Biochemistry

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